This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. 3. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. Methods. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Plaque Reduction Neutralization Test (PRNT) SARS-CoV-2 neutralization was assessed as previously described . Cet appareil ISM est confomre à la norme NMB-001 du Canada. Spectranet customer portal. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. . The e ect of ASC-exosomes on the melanin levels inThe 70% methanolic extract of steamed root (100 µL) was added to 1 mL of diethylene glycol and 10 µL of sodium hydroxide (NaOH). Bioz Stars score: 98/100, based on 1 PubMed citations. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Bulk Kit. ; SoftMax® Pro 7. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. . **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). applications, including dual luciferase and ATP assays. . SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The CellTiter-Glo assay from Promega uses luciferase. 1. Reporter Assay. The dual auto injector module. 303 unable to cal dark current. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Controls containing all reaction. 99. 1. Molecular Devicesの全商品を見る. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. The protocols are also available on the SoftMax Pro Software protocol sharing. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. ü; ü. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Citation 2002). Danaher Inc spectramax i3x. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. GM3000 Register or Sign In. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Structured Review. SpectraMax® ABS and ABS Plus reader support; ü. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 315 can't find zero order. Prepare your i3/i3x instrument for shipment. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Version 7. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Product Details. The Results. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. Expand your lab’s capabilities to include flash applications such as dual luciferase and. ATP (“flash” luminescence using . Molecular Devices are known for both the reliability and the wide uses of their products. 660-665 Eskdale Road. The information is below the horizontal line. Description 1. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. 99 to $1999. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. SpectraMax i3x Multi-Mode Detection Platform User Guide. 2. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. compound concentration, and an IC 50 value of 21. SpectraMax ® i3/i3x Multi-Mode Microplate Reader; SpectraMax Plus 384 Absorbance Microplate Reader; Methods. Price from $9. 0. . Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. Spectranet customer portalThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. Seller Details. 1. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Examples: “Press carefully to engage,” “ Do not. Find our technical support information along with contact details. . Download PDF. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax i3x, onboard luminescence 121. Prepare your iD series instrument for shipment. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Use the 3. Pick the best speed, sensitivity, and price for your needs. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. SpectraMax M5. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. Summary of Contents for Molecular Devices SpectraMax M2. 0. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Multimode Reader Comparison Chart. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. The assay can accurately test the toxicology of compounds as shown in Figure 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Explore a wealth of applications with the future-ready SpectraMax i3x. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 0´ 104 4. 2 x 10-14. 58% for the detection module and 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. 吸収度マイクロプレートリーダー SpectraMax® Mini. Download PDF. Not compatible with Home Edition. Description 1. Figure 4. 003 fmol 0. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Cell viability는 세포막 Integrity 또는. 3) At the bottom of the strip, click on "Show. 5 decades, with sensitive detection of cells. 1 and above:. SpectraMax ® iD3 and iD5 Multi-Mode . The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. . The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. Find regional support. 1. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. See Unpacking the SpectraMax Paradigm Instrument on page 1. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Average 98 stars, based on 1 article reviews. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. We would like to show you a description here but the site won’t allow us. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. 3 Figures from 612 Publications Promega. Introduction. For wavelengths in the UV range above 220 nm,. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. . Register to download your free eBook today. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. EMax Plus Microplate Reader. Rutin was used to generate standard curves for quantification. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. Find regional support. 5 Figures from 202 Publications Thermo Fisher Scientific. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. com. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. TaqMan SNP. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Average 98 stars, based on 1 article reviews. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Instrument. . HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax i3x Multi-Mode Detection Platform. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Serial number 33 900 01-3387. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Ease of use. 5577 Web: Email: info@moldev. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. User-upgradeable detection modules allow you to expand the capabilities of your microp. Microplate optimization should be performed the first time the assay is run, and subsequently for . Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. . 2 only) SpectraMax M-series Microplate readers. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The calculated LOQ values were 0. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. Features. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. Explore PDF for more details. Bioz Stars score: 98/100, based on 1 PubMed citations. Figure 2-23: Bottle holder To install the bottle holder: 1. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Check software compatibility with Windows 10 and locate your software installer. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Microplate reader assays requiring infrared (IR) or red-shifted. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. QuickSync. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. . Supported Windows Operating System: See release notes. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Data Analysis. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 在这里，我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Description 1. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. 0´ 104 1. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The corrected absorbance is within 5% of the. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. It allows you to explore cellular pathways and protein activation and expression in one system. to View Figures. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Controls containing all reaction. 3: The cuvette chamber. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. The chamber. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Italic and boldface type are used for emphasis. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. . The treatments were. com. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 304 signal level saturation. . Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. 7 Figures from 1126 Publications. The plate was shaken and incubated in the dark for 10 minutes. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. It allows you to explore cellular pathways and protein activation and expression in one system. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Open the catalog to page 2. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. 1. Downloads. Calcium. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. Explore a wealth of applications with the future-ready SpectraMax i3x. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Monday to Friday 7am - 5pm Pacific Time. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. . Nuclear Receptor. . EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. . Simplified workflow: Add cellular imaging assays in a small footprint. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The plate was shaken and incubated in the dark for 10 minutes. . Example: [Drawer]. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. D 1 1. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Recommended settings for SpectraMax iD5 and i3x readers. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 7 Figures from 1126 Publications Promega. Bioz Stars score: 98/100, based on 1 PubMed citations. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. We would like to show you a description here but the site won’t allow us. . Products : SoftMax Pro GxP Software, SoftMax Pro Software. • Removing the transport locks from the PMT shutters and drawers. Restart the system. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). 9 x 10-15. iD5. user upgradeable. Our absorbance plate readers feature our PathCheck Sensor. Find our technical support information along with contact details. . 価格、見積もり、お近くの販売店を. Wokingham, Berkshire RG41 5TS United Kingdom. Description. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Danaher Inc spectramax i3x. EUROPE. 1. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. The SpectraMax® i3x reader allows you to. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. *On the SpectraMax i3x reader only. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. ; The top 3 importers of Spectramax are India with 365. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. +44-118-944-8000. Request a Quote for This Setup. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. ZERO BIAS - scores, article reviews, protocol conditions and more. i3x. Download SoftMax® Pro 7. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. pH-Xtra Glycolysis Assay - Agilent. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Prepare your i3/i3x instrument for shipment.